AKSF1 isolated from the rice-virulent Acidovorax avenae K1 strain is a novel effector that suppresses PAMP-triggered immunity in rice.
Identifieur interne : 000222 ( Main/Exploration ); précédent : 000221; suivant : 000223AKSF1 isolated from the rice-virulent Acidovorax avenae K1 strain is a novel effector that suppresses PAMP-triggered immunity in rice.
Auteurs : Takemasa Kawaguchi [Japon] ; Minami Nakamura [Japon] ; Hiroyuki Hirai [Japon] ; Takehito Furukawa [Japon] ; Machiko Kondo [Japon] ; Fang-Sik Che [Japon]Source :
- Molecular plant-microbe interactions : MPMI [ 0894-0282 ] ; 2020.
Abstract
Microbial pathogens deliver effectors into plant cells to suppress plant immune responses and modulate host metabolism in order to support infection processes. We sought to determine if the Acidovorax avenae rice-virulent K1 strain can suppress pathogen-associated molecular pattern (PAMP)-triggered immunity (PTI) induced by flagellin isolated from the rice-avirulent N1141 strain. The flagellin-triggered PTI, including H2O2 generation, callose deposition, and expression of several immune-related genes were strongly suppressed in K1 pre-inoculated cultured rice cells in a Type III secretion system (T3SS)-dependent manner. By screening 4,562 transposon-tagged mutants based on their suppression ability, we found that 156 transposon-tagged K1 mutants lost the ability to suppress PTI induction. Mutant sequence analysis, comprehensive expression analysis using RNA-sequencing, and the prediction of secretion through T3SS showed that a protein named A. avenae K1 suppression factor 1 (AKSF1) suppresses flagellin-triggered PTI in rice. Translocation of AKSF1 protein into rice cells is dependent on T3SS during infection, an AKSF1-disruption mutant lost the ability to suppress PTI responses, and expression of AKSF1 in AKSF1-disruption mutant complemented the suppression activity. When AKSF1-disruption mutants were inoculated into the host rice plant, reduction of the disease symptoms and suppression of the bacterial growth were observed. Taken together, our results demonstrate that AKSF1 is a novel effector that can suppress the PTI in host rice plant.
DOI: 10.1094/MPMI-10-20-0271-R
PubMed: 33135963
Affiliations:
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b113129@m.nagahama-i-bio.ac.jp.</nlm:affiliation><country wicri:rule="url">Japon</country><wicri:regionArea>Nagahama Institute of Bio-Science and Technology, 53362, Graduate School of Biosciences, Nagahama, Shiga</wicri:regionArea><wicri:noRegion>Shiga</wicri:noRegion></affiliation></author><author><name sortKey="Hirai, Hiroyuki" sort="Hirai, Hiroyuki" uniqKey="Hirai H" first="Hiroyuki" last="Hirai">Hiroyuki Hirai</name><affiliation wicri:level="1"><nlm:affiliation>Nagahama Institute of Bio-Science and Technology, 53362, Department of Bio-Science, Nagahama, Shiga, Japan; h_hirai@nagahama-i-bio.ac.jp.</nlm:affiliation><country wicri:rule="url">Japon</country><wicri:regionArea>Nagahama Institute of Bio-Science and Technology, 53362, Department of Bio-Science, Nagahama, Shiga</wicri:regionArea><wicri:noRegion>Shiga</wicri:noRegion></affiliation></author><author><name sortKey="Furukawa, Takehito" sort="Furukawa, Takehito" uniqKey="Furukawa T" first="Takehito" last="Furukawa">Takehito Furukawa</name><affiliation wicri:level="1"><nlm:affiliation>Nagahama Institute of Bio-Science and Technology, 53362, Department of Bio-Science, Tamura 1266, Nagahama, Shiga, Japan, 526-0829; 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k_sai@nagahama-i-bio.ac.jp.</nlm:affiliation><country wicri:rule="url">Japon</country><wicri:regionArea>Nagahama Institute of Bio-Science and Technology Department of Bioscience Graduate School of Bioscience, 265247, Nagahama, Shiga</wicri:regionArea><wicri:noRegion>Shiga</wicri:noRegion></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2020">2020</date><idno type="RBID">pubmed:33135963</idno><idno type="pmid">33135963</idno><idno type="doi">10.1094/MPMI-10-20-0271-R</idno><idno type="wicri:Area/Main/Corpus">000025</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000025</idno><idno type="wicri:Area/Main/Curation">000025</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">000025</idno><idno type="wicri:Area/Main/Exploration">000025</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">AKSF1 isolated from the rice-virulent <i>Acidovorax avenae</i> K1 strain is a novel effector that suppresses PAMP-triggered immunity in rice.</title><author><name sortKey="Kawaguchi, Takemasa" sort="Kawaguchi, Takemasa" uniqKey="Kawaguchi T" first="Takemasa" last="Kawaguchi">Takemasa Kawaguchi</name><affiliation wicri:level="1"><nlm:affiliation>Nagahama Institute of Bio-Science and Technology, 53362, Department of Bio-Science, Nagahama, Shiga, Japan; b110039@nagahama-i-bio.ac.jp.</nlm:affiliation><country wicri:rule="url">Japon</country><wicri:regionArea>Nagahama Institute of Bio-Science and Technology, 53362, Department of Bio-Science, Nagahama, Shiga</wicri:regionArea><wicri:noRegion>Shiga</wicri:noRegion></affiliation></author><author><name sortKey="Nakamura, Minami" sort="Nakamura, Minami" uniqKey="Nakamura M" first="Minami" last="Nakamura">Minami Nakamura</name><affiliation wicri:level="1"><nlm:affiliation>Nagahama Institute of Bio-Science and Technology, 53362, Graduate School of Biosciences, Nagahama, Shiga, Japan; b113129@m.nagahama-i-bio.ac.jp.</nlm:affiliation><country wicri:rule="url">Japon</country><wicri:regionArea>Nagahama Institute of Bio-Science and Technology, 53362, Graduate School of Biosciences, Nagahama, Shiga</wicri:regionArea><wicri:noRegion>Shiga</wicri:noRegion></affiliation></author><author><name sortKey="Hirai, Hiroyuki" sort="Hirai, Hiroyuki" uniqKey="Hirai H" first="Hiroyuki" last="Hirai">Hiroyuki Hirai</name><affiliation wicri:level="1"><nlm:affiliation>Nagahama Institute of Bio-Science and Technology, 53362, Department of Bio-Science, Nagahama, Shiga, Japan; h_hirai@nagahama-i-bio.ac.jp.</nlm:affiliation><country wicri:rule="url">Japon</country><wicri:regionArea>Nagahama Institute of Bio-Science and Technology, 53362, Department of Bio-Science, Nagahama, Shiga</wicri:regionArea><wicri:noRegion>Shiga</wicri:noRegion></affiliation></author><author><name sortKey="Furukawa, Takehito" sort="Furukawa, Takehito" uniqKey="Furukawa T" first="Takehito" last="Furukawa">Takehito Furukawa</name><affiliation wicri:level="1"><nlm:affiliation>Nagahama Institute of Bio-Science and Technology, 53362, Department of Bio-Science, Tamura 1266, Nagahama, Shiga, Japan, 526-0829; t_furukawa@nagahama-i-bio.ac.jp.</nlm:affiliation><country wicri:rule="url">Japon</country><wicri:regionArea>Nagahama Institute of Bio-Science and Technology, 53362, Department of Bio-Science, Tamura 1266, Nagahama, Shiga, Japan</wicri:regionArea><wicri:noRegion>Japan</wicri:noRegion></affiliation></author><author><name sortKey="Kondo, Machiko" sort="Kondo, Machiko" uniqKey="Kondo M" first="Machiko" last="Kondo">Machiko Kondo</name><affiliation wicri:level="1"><nlm:affiliation>Nagahama Institute of Bio-Science and Technology, 53362, Department of Bio-Science, Nagahama, Shiga, Japan; m_kondo@nagahama-i-bio.ac.jp.</nlm:affiliation><country wicri:rule="url">Japon</country><wicri:regionArea>Nagahama Institute of Bio-Science and Technology, 53362, Department of Bio-Science, Nagahama, Shiga</wicri:regionArea><wicri:noRegion>Shiga</wicri:noRegion></affiliation></author><author><name sortKey="Che, Fang Sik" sort="Che, Fang Sik" uniqKey="Che F" first="Fang-Sik" last="Che">Fang-Sik Che</name><affiliation wicri:level="1"><nlm:affiliation>Nagahama Institute of Bio-Science and Technology, 53362, Graduate School of Bio-Science, Nagahama, Shiga, Japan.</nlm:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>Nagahama Institute of Bio-Science and Technology, 53362, Graduate School of Bio-Science, Nagahama, Shiga</wicri:regionArea><wicri:noRegion>Shiga</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>Nagahama Institute of Bio-Science and Technology Department of Bioscience Graduate School of Bioscience, 265247, Nagahama, Shiga, Japan; k_sai@nagahama-i-bio.ac.jp.</nlm:affiliation><country wicri:rule="url">Japon</country><wicri:regionArea>Nagahama Institute of Bio-Science and Technology Department of Bioscience Graduate School of Bioscience, 265247, Nagahama, Shiga</wicri:regionArea><wicri:noRegion>Shiga</wicri:noRegion></affiliation></author></analytic><series><title level="j">Molecular plant-microbe interactions : MPMI</title><idno type="ISSN">0894-0282</idno><imprint><date when="2020" type="published">2020</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Microbial pathogens deliver effectors into plant cells to suppress plant immune responses and modulate host metabolism in order to support infection processes. We sought to determine if the <i>Acidovorax avenae</i> rice-virulent K1 strain can suppress pathogen-associated molecular pattern (PAMP)-triggered immunity (PTI) induced by flagellin isolated from the rice-avirulent N1141 strain. The flagellin-triggered PTI, including H<sub>2</sub>O<sub>2</sub> generation, callose deposition, and expression of several immune-related genes were strongly suppressed in K1 pre-inoculated cultured rice cells in a Type III secretion system (T3SS)-dependent manner. By screening 4,562 transposon-tagged mutants based on their suppression ability, we found that 156 transposon-tagged K1 mutants lost the ability to suppress PTI induction. Mutant sequence analysis, comprehensive expression analysis using RNA-sequencing, and the prediction of secretion through T3SS showed that a protein named <i>A. avenae</i> K1 suppression factor 1 (AKSF1) suppresses flagellin-triggered PTI in rice. Translocation of AKSF1 protein into rice cells is dependent on T3SS during infection, an <i>AKSF1</i>-disruption mutant lost the ability to suppress PTI responses, and expression of <i>AKSF1</i> in <i>AKSF1</i>-disruption mutant complemented the suppression activity. When <i>AKSF1</i>-disruption mutants were inoculated into the host rice plant, reduction of the disease symptoms and suppression of the bacterial growth were observed. Taken together, our results demonstrate that AKSF1 is a novel effector that can suppress the PTI in host rice plant.</div></front></TEI><pubmed><MedlineCitation Status="Publisher" Owner="NLM"><PMID Version="1">33135963</PMID><DateRevised><Year>2020</Year><Month>11</Month><Day>02</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Print">0894-0282</ISSN><JournalIssue CitedMedium="Print"><PubDate><Year>2020</Year><Month>Nov</Month><Day>02</Day></PubDate></JournalIssue><Title>Molecular plant-microbe interactions : MPMI</Title><ISOAbbreviation>Mol Plant Microbe Interact</ISOAbbreviation></Journal><ArticleTitle>AKSF1 isolated from the rice-virulent <i>Acidovorax avenae</i> K1 strain is a novel effector that suppresses PAMP-triggered immunity in rice.</ArticleTitle><ELocationID EIdType="doi" ValidYN="Y">10.1094/MPMI-10-20-0271-R</ELocationID><Abstract><AbstractText>Microbial pathogens deliver effectors into plant cells to suppress plant immune responses and modulate host metabolism in order to support infection processes. We sought to determine if the <i>Acidovorax avenae</i> rice-virulent K1 strain can suppress pathogen-associated molecular pattern (PAMP)-triggered immunity (PTI) induced by flagellin isolated from the rice-avirulent N1141 strain. The flagellin-triggered PTI, including H<sub>2</sub>O<sub>2</sub> generation, callose deposition, and expression of several immune-related genes were strongly suppressed in K1 pre-inoculated cultured rice cells in a Type III secretion system (T3SS)-dependent manner. By screening 4,562 transposon-tagged mutants based on their suppression ability, we found that 156 transposon-tagged K1 mutants lost the ability to suppress PTI induction. Mutant sequence analysis, comprehensive expression analysis using RNA-sequencing, and the prediction of secretion through T3SS showed that a protein named <i>A. avenae</i> K1 suppression factor 1 (AKSF1) suppresses flagellin-triggered PTI in rice. Translocation of AKSF1 protein into rice cells is dependent on T3SS during infection, an <i>AKSF1</i>-disruption mutant lost the ability to suppress PTI responses, and expression of <i>AKSF1</i> in <i>AKSF1</i>-disruption mutant complemented the suppression activity. When <i>AKSF1</i>-disruption mutants were inoculated into the host rice plant, reduction of the disease symptoms and suppression of the bacterial growth were observed. Taken together, our results demonstrate that AKSF1 is a novel effector that can suppress the PTI in host rice plant.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Kawaguchi</LastName><ForeName>Takemasa</ForeName><Initials>T</Initials><AffiliationInfo><Affiliation>Nagahama Institute of Bio-Science and Technology, 53362, Department of Bio-Science, Nagahama, Shiga, Japan; b110039@nagahama-i-bio.ac.jp.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Nakamura</LastName><ForeName>Minami</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>Nagahama Institute of Bio-Science and Technology, 53362, Graduate School of Biosciences, Nagahama, Shiga, Japan; b113129@m.nagahama-i-bio.ac.jp.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Hirai</LastName><ForeName>Hiroyuki</ForeName><Initials>H</Initials><AffiliationInfo><Affiliation>Nagahama Institute of Bio-Science and Technology, 53362, Department of Bio-Science, Nagahama, Shiga, Japan; h_hirai@nagahama-i-bio.ac.jp.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Furukawa</LastName><ForeName>Takehito</ForeName><Initials>T</Initials><AffiliationInfo><Affiliation>Nagahama Institute of Bio-Science and Technology, 53362, Department of Bio-Science, Tamura 1266, Nagahama, Shiga, Japan, 526-0829; t_furukawa@nagahama-i-bio.ac.jp.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Kondo</LastName><ForeName>Machiko</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>Nagahama Institute of Bio-Science and Technology, 53362, Department of Bio-Science, Nagahama, Shiga, Japan; m_kondo@nagahama-i-bio.ac.jp.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Che</LastName><ForeName>Fang-Sik</ForeName><Initials>FS</Initials><AffiliationInfo><Affiliation>Nagahama Institute of Bio-Science and Technology, 53362, Graduate School of Bio-Science, Nagahama, Shiga, Japan.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Nagahama Institute of Bio-Science and Technology Department of Bioscience Graduate School of Bioscience, 265247, Nagahama, Shiga, Japan; k_sai@nagahama-i-bio.ac.jp.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2020</Year><Month>11</Month><Day>02</Day></ArticleDate></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Mol Plant Microbe Interact</MedlineTA><NlmUniqueID>9107902</NlmUniqueID><ISSNLinking>0894-0282</ISSNLinking></MedlineJournalInfo><CitationSubset>IM</CitationSubset></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="entrez"><Year>2020</Year><Month>11</Month><Day>2</Day><Hour>12</Hour><Minute>9</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2020</Year><Month>11</Month><Day>3</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2020</Year><Month>11</Month><Day>3</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>aheadofprint</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">33135963</ArticleId><ArticleId IdType="doi">10.1094/MPMI-10-20-0271-R</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Japon</li></country></list><tree><country name="Japon"><noRegion><name sortKey="Kawaguchi, Takemasa" sort="Kawaguchi, Takemasa" uniqKey="Kawaguchi T" first="Takemasa" last="Kawaguchi">Takemasa Kawaguchi</name></noRegion><name sortKey="Che, Fang Sik" sort="Che, Fang Sik" uniqKey="Che F" first="Fang-Sik" last="Che">Fang-Sik Che</name><name sortKey="Che, Fang Sik" sort="Che, Fang Sik" uniqKey="Che F" first="Fang-Sik" last="Che">Fang-Sik Che</name><name sortKey="Furukawa, Takehito" sort="Furukawa, Takehito" uniqKey="Furukawa T" first="Takehito" last="Furukawa">Takehito Furukawa</name><name sortKey="Hirai, Hiroyuki" sort="Hirai, Hiroyuki" uniqKey="Hirai H" first="Hiroyuki" last="Hirai">Hiroyuki Hirai</name><name sortKey="Kondo, Machiko" sort="Kondo, Machiko" uniqKey="Kondo M" first="Machiko" last="Kondo">Machiko Kondo</name><name sortKey="Nakamura, Minami" sort="Nakamura, Minami" uniqKey="Nakamura M" first="Minami" last="Nakamura">Minami Nakamura</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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